Regulatory
Part:BBa_K1484334
Designed by: Angelina Munabi Group: iGEM14_Cambridge-JIC (2014-10-10)
P_NiI19, marchantia promoter
This part is an untested potential promoter for Marchantia polymorpha. It is thought to initiate transcription of a nitrate transporter protein under conditions of limiting nitrate (NO-3). We identified the promoter during a broader search for potential inputs to our genetic circuit, and we think it may be used as such, but testing will definitely be required to confirm this. We submit this part as a foundation for future marchantia work. We were able to physically obtain DNA from a PCR of a marchantia genome.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 644
Illegal SpeI site found at 722 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1570
Illegal SpeI site found at 644
Illegal SpeI site found at 722 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1847
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 644
Illegal SpeI site found at 722 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 644
Illegal SpeI site found at 722 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1678
Illegal BsaI.rc site found at 508
[edit]
Categories
Parameters
//promoter
None |